Sunday, September 20, 2020

Animal Cell Culture 5marks question, Class 12 (BIOTECH)

 

  1. Why the technique for the production of monoclonal antibodies called hybridoma technology?  Why monoclonal antibodies are preferred over serum antibodies in diagnostics? Give an example of a therapeutic monoclonal antibody for breast cancer patients.
  2. A child suffering from acute lymphocytic leukemia underwent a bone marrow replacement therapy wherein her bone marrow was destroyed and replaced by bone marrow obtained from a sibling who was normal. Why was the bone marrow destroyed during the therapy? Do you expect the child to recover? Explain.
  3. Embryonic cells during development not only commit along different lineages but also retain a population of cells are present only at strategic locations in the adult organism. Name these specialized cells and why they are maintained in undifferentiated state?
  4. What is OKT-3? Why is it administered to patients undergoing organ transplantation? What is the relevance of fusing an antibody producing B-cells with myeloma cells in hybridoma technology?
  5. (a) Discuss about the method involved in the scaling up of the suspension culture. (b) Imagine yourself as a G.E. and had produced a recombinant molecule carrying gene of interest i.e. tPA. You want to propagate it. Suggest suitable method for the same.
  6. What are the medical application of the following:

(i) tPA   (ii) Monoclonal Antibodies    (iii) Erythropoietin  (iv) Factor VIII     (v) Factor IX

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