- Why the technique for the production of monoclonal antibodies called hybridoma technology? Why monoclonal antibodies are preferred over serum antibodies in diagnostics? Give an example of a therapeutic monoclonal antibody for breast cancer patients.
- A child suffering from acute lymphocytic leukemia underwent a bone marrow replacement therapy wherein her bone marrow was destroyed and replaced by bone marrow obtained from a sibling who was normal. Why was the bone marrow destroyed during the therapy? Do you expect the child to recover? Explain.
- Embryonic cells during development not only commit along different lineages but also retain a population of cells are present only at strategic locations in the adult organism. Name these specialized cells and why they are maintained in undifferentiated state?
- What is OKT-3? Why is it administered to patients undergoing organ transplantation? What is the relevance of fusing an antibody producing B-cells with myeloma cells in hybridoma technology?
- (a) Discuss about the method involved in the scaling up of the suspension culture. (b) Imagine yourself as a G.E. and had produced a recombinant molecule carrying gene of interest i.e. tPA. You want to propagate it. Suggest suitable method for the same.
- What are the medical application of the following:
(i) tPA (ii) Monoclonal Antibodies (iii) Erythropoietin (iv) Factor VIII (v) Factor IX
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