Animal Cell Culture 3mark questions, Class 12(BIOTECH)

 

1. What is OKT-3? Why is it administered to patients undergoing organ transplantation? What is the relevance of fusing an antibody producing B-cells with myeloma cells in hybridoma technology?
2. If you wish to scale up cells derived from human tissue, what kind of culture set up will you use and why?
3. (a) What is stem cell? (b) Explain erythropoiesis with suitable diagram (c) What is the possible benefit of embryonic stem cell technology?
4. Embryonic cells during development not only commit along different lineages but also retain a population of cells are present only at strategic locations in the adult organism. Name these specialized cells and why they are maintained in undifferentiated state?
5. ES cells could potentially be used for treatment of variety diseases associated with tissue damage? Defend this statement by giving three examples of ES therapeutics.
6. Why is humulin considered better than pig insulin for the treatment of diabetes?
7. Indicate three applications of stem cell technology.
8. Stem cell technology is potentially very exciting. Why? Give three sources of stem cell?
9. Outline the process of creation of chimeric mouse by embryonic stem cell culture.
10. Explain the methods which can be used for the scaling up of animal culture.
11. (a) Differentiate between stem cells and progenitor cells. (b) Name two sources of stem cells.
12. (a) Stem Cell technology is potentially very exciting. Why? (b)Why do we retain stem cells throughout adult life?
13. Describe the use of the following in an animal cell culture laboratory: (a) LAF hood (b) Inverted microscope (c) Microcarrier beads