RECOMBINANT DNA TECHNOLOGY, CLASS 12 (WORKSHEET 1)

RECOMBINANT DNA TECHNOLOGY, CLASS 12 

TOOLS IN RDT

1. Give two reasons why different vectors are required for recombinant DNA technology?
2. What are expression vectos? What kind of promoters should be used in such vectors?
3. Why are restriction enzymes called 'molecular scissors' or 'molecular scalpels'? Indicate the properties of these enzymes highlighting their role in RDT. Describe the nomenclature of restriction endonuclease.
4. Name any two selectable markers.
5. What are the properties of an ideal vector/
6. Which enzyme is used to prevent self ligation?
7. Define gene cloning.
8. Describe about the basic steps involved in RDT and illustrate them schematically.
9. Name the scientist who had generated first rDNA.
10. Why does restriction endonuclease exist in many bacterias? How does it protect its own DNA from the attack of restriction endonuclease. Illustrate with the help of diagram.
11. What is the function of methylase?
12. Why only type II restriction endonuclease are used in RDT?
13. Who are responsible for the discovery of first R.E.?
14. Write the recognition sequence and microbial source of these restriction enzyme -  Alu I, EcoRI, Bam HI, HaeIII.
15. Differentiate between staggered cuts and symmetrical cuts.
16. Write a note on RFLP.
17. What are the sources of DNA ligase and Alkaline phoshatase?
18. Differentiate between: a. pBR and pUC
                                           b. M13 and lambda phage
19. Draw a neat and labelled diagram of pBR, pUC, Yep vectors
20. What is the significance of cos site?