WORKSHEET 2 RDT

RECOMBINANT DNA TECHNOLOGY, CLASS 12 (WORKSHEET 2)

RECOMBINANT DNA TECHNOLOGY, CLASS 12 

( Host cell and Introduction of rDNA into host cell)

1. Why E.coli is most preffered host for propogation of rDNA?

2. Why we can't use E.coli for the expression of prokaryotic proteins? What alternative can we use for this? Justify.

3. With a diagrammatic explanation, show making of recombinant plasmid.

4. Discuss in detail about any five methods of introducing rDNA into host cell. Draw suitable diagram also. (Large Answer type question).

5. Name the scientist who found that E.coli cells become competent  upon calcium chloride treatment.