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1. For the expression of eukaryotic proteins, eukaryotic cells are often preferred:
- all of these
2. In transfection foreign DNA is not mixed with
- anionic liposomes, all other are positively charged
3. This method is mainly used for introduction of foreign DNA into plant cells
- particle gun bombardment
4. PCR can't be used for
- baking the DNA fragments
5. In agarose gel electrophersis, the fragment which is obtained at the top is
- 15640 Da, as it is having highest molecular weight
6. On what principle Southern Hybridization is based. (1)
- Hybridization i.e. ability of probe to seek out and bind to its complementary sequences
7. What is the principle behind PCR? (1)
- the basic underlying principle is that when a double stranded DNA is heated to a high temperature, separated single stranded DNA can be made to hybridize with primers by bringing down teperature and then polymerase do extension resulting in amplification of DNA.
8. Why Yeast is most preffered host among eukaryotes? (2)
- Pg 13 text book Hotst Cell (3rd paragraph)
9. What is electroporation?
- a method used for introduction of rDNA into host cells by the use of electric field creating transient pores.
10. What are the basic requirements for PCR? (3)
- DNA template, primers, Thermostable DNA polymerase, dNTPS, magnesium ions and buffer
11. What is probe? How it can be used for visualization? (2)
- Probes are relatively small single stranded sequences of DNA that recognizes and bind to complementary sequence. They are tagged eith fluorescent label or radioactive label for identification.
12. What is blotting?
- Transfer of DNA fragments from gel to a nylon or nitrocellullose membrane.
1. For the expression of eukaryotic proteins, eukaryotic cells are often preferred:
- all of these
2. In transfection foreign DNA is not mixed with
- anionic liposomes, all other are positively charged
3. This method is mainly used for introduction of foreign DNA into plant cells
- particle gun bombardment
4. PCR can't be used for
- baking the DNA fragments
5. In agarose gel electrophersis, the fragment which is obtained at the top is
- 15640 Da, as it is having highest molecular weight
6. On what principle Southern Hybridization is based. (1)
- Hybridization i.e. ability of probe to seek out and bind to its complementary sequences
7. What is the principle behind PCR? (1)
- the basic underlying principle is that when a double stranded DNA is heated to a high temperature, separated single stranded DNA can be made to hybridize with primers by bringing down teperature and then polymerase do extension resulting in amplification of DNA.
8. Why Yeast is most preffered host among eukaryotes? (2)
- Pg 13 text book Hotst Cell (3rd paragraph)
9. What is electroporation?
- a method used for introduction of rDNA into host cells by the use of electric field creating transient pores.
10. What are the basic requirements for PCR? (3)
- DNA template, primers, Thermostable DNA polymerase, dNTPS, magnesium ions and buffer
11. What is probe? How it can be used for visualization? (2)
- Probes are relatively small single stranded sequences of DNA that recognizes and bind to complementary sequence. They are tagged eith fluorescent label or radioactive label for identification.
12. What is blotting?
- Transfer of DNA fragments from gel to a nylon or nitrocellullose membrane.
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