1. Differentiate between synthetic and complex medium used for microbial culture.
2. How does the metagenomics help in identification of novel genes present in the environment? Explain the process.
3. Suggest any three advantages of using Pichia pastoris as a eukaryotic expression.
4. What are the essential steps involved in the isolation of vitamin B12 from the microbe?
5. (a) Calculate the generation time of a bacterial population in which the number of bacteria increases from 104 / ml to 107/ml furing four hours of exponential growth.
(b) Explain any two methods of measuring microbial growth.
(c) In which phase of growth is the specific growth rate of microbial cells calculated? On what factors does the specific growth rate depend?
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