PRACTICE WORKSHEET OF GENOMICS (Introduction, GenePrediction, SNP, FISH)

1. Define SNPs. Describe a possible use of this technique in medicine. How do the physicians decide our susceptibility or resistance to a particular disease through this technique? Explain with the help of an example.

2. Gene prediction by computers is different from number of genes identified by experimental methods. Why is it so? Is there any correlation between the complexity of an organism and the total number of genes in its genome? Justify.

3. Name the two methodologies used for genome sequencing. Present a comparative account.

4. CML patients have the ‘Philadelphia Chromosome”. How is this chromosome detected? Explain the technique with a diagram.

5. A  Chronic Myelogenous Leukemia (CML) patient has been put on a combination drug therapy for the past 2 months. How can the FISH technique be used to monitor the effect of chemotherapy?

6. Based on Genomic studies, why do people say that different species and organisms had a common ancestor 100 million years ago.    

7. There is no simple correlation between the intuitive complexity of an organism and the number of genes in its genome. Do you agree with the statement? Justify your answer.

8. It is believed that each person’s genome is 99.8% is identical to everyone else’s. Discuss the basis of this 0.2% difference in DNA sequence of  individuals. Does it always occur in coding regions of the genome? Suggest the usefulness of studying such variations using two examples.

9. How are fluorescent colors introduced into chromosomes? Give a possible use of this technique. Draw a suitable diagram of the same

10. (a) Human population vary greatly in their susceptibility to diseases. Why?

(b)Which present day approach can assist physicians in predicting with confidence the risk of developing a disease.