1. How are genomic libraries different from cDNA libraries? Schematically depict the various steps involved in generating a genomic library? (5)
2. How will you select bacterial cells carrying a recombinant plasmid? (3)
3. How Lac Z gene can be used as a screening marker? Name one another selectable marker. (2)
4.Why cDNA is preferred over genomic DNA in molecular researches? (2)
5. Explain the role of insertional inactivation in rDNA technology? (5)
6. Explain briefly a technique for visual screening of transformed bacteria. (2)
7. An unusual plasmid has been discovered which has high transformation ability in E.coli and confers heat resistance to the host. Hence the host E.coli when transformed with this plasmid can grow at 50 degree celsius. Schematically represent the various steps of cloning a foreign gene in this plasmid. How would you screen the transformed E.coli carrying this plasmid? (3)
2. How will you select bacterial cells carrying a recombinant plasmid? (3)
3. How Lac Z gene can be used as a screening marker? Name one another selectable marker. (2)
4.Why cDNA is preferred over genomic DNA in molecular researches? (2)
5. Explain the role of insertional inactivation in rDNA technology? (5)
6. Explain briefly a technique for visual screening of transformed bacteria. (2)
7. An unusual plasmid has been discovered which has high transformation ability in E.coli and confers heat resistance to the host. Hence the host E.coli when transformed with this plasmid can grow at 50 degree celsius. Schematically represent the various steps of cloning a foreign gene in this plasmid. How would you screen the transformed E.coli carrying this plasmid? (3)
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